PrimePCR™ SYBR® Green Assay: TEAD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055146

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047886
Assay Design:   exonic
Chromosome Location:   11:12959380-12959509question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000527636 ENST00000527575 ENST00000526600 ENST00000361985 ENST00000361905 ENST00000334310

Gene Information

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and in placental cells also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq May 2010]

Gene Symbol:   TEAD1
Gene Name:   TEA domain family member 1 (SV40 transcriptional enhancer factor)
Aliases:   AA, FLJ17970, NTEF-1, REF1, TCF-13, TCF13, TEAD-1, TEF-1
RefSeq:   NC_000011.9 NG_021302.1 NT_009237.18
Ensembl:   ENSG00000187079
Entrez:   7003
Chromosome Mapping:   11p15.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.310000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download