PrimePCR™ SYBR® Green Assay: TMEM232, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055138

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TMEM232, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TMEM232, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047878
Assay Design:   exonic
Chromosome Location:   5:109976600-109976696question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000379939 ENST00000379922 ENST00000540320 ENST00000400445 ENST00000310336 ENST00000373233 ENST00000309279 ENST00000460680 ENST00000469613 ENST00000296288 ENST00000478368 ENST00000362026 ENST00000545908 ENST00000216605 ENST00000555709 ENST00000268616 ENST00000561928 ENST00000368237 ENST00000340086 ENST00000413282 ENST00000368236 ENST00000455884 ENST00000511883 ENST00000515278 ENST00000512886 ENST00000429839 ENST00000541158 ENST00000298171

Gene Information

Description Not Available

Gene Symbol:   TMEM232
Gene Name:   transmembrane protein 232
Aliases:   DKFZp686G0585, FLJ35903, FLJ43080
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000186952
Entrez:   642987
Chromosome Mapping:   5q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 46.730000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download