PrimePCR™ SYBR® Green Assay: MAPT, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055132

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047872
Assay Design:   exonic
Chromosome Location:   17:44073875-44074014question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000549053 ENST00000550769 ENST00000447599 ENST00000549598 ENST00000550493 ENST00000553101 ENST00000546644 ENST00000547239 ENST00000552530 ENST00000552974 ENST00000308595 ENST00000334239 ENST00000446361 ENST00000420682 ENST00000415613 ENST00000571987 ENST00000574436 ENST00000431008 ENST00000576518 ENST00000344290 ENST00000262410 ENST00000351559 ENST00000340799 ENST00000535772 ENST00000347967

Gene Information

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex regulated alternative splicing giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease Pick's disease frontotemporal dementia cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq Jul 2008]

Gene Symbol:   MAPT
Gene Name:   microtubule-associated protein tau
Aliases:   DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU
RefSeq:   NC_000017.10 NT_010783.15 NT_167251.1 NG_007398.1
Ensembl:   ENSG00000186868
Entrez:   4137
UniGene:   Hs.101174
Chromosome Mapping:   17q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.120000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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