PrimePCR™ SYBR® Green Assay: PABPC1L2A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055080

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047820
Assay Design:   exonic
Chromosome Location:   X:72298796-72298945question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000357949 ENST00000462284 ENST00000539479 ENST00000393415 ENST00000393416 ENST00000350057 ENST00000398665 ENST00000440640 ENST00000310127 ENST00000525591 ENST00000539485 ENST00000379573 ENST00000379567 ENST00000246041 ENST00000373521 ENST00000538388 ENST00000373519 ENST00000453389 ENST00000534978 ENST00000242108 ENST00000600221 ENST00000599154 ENST00000599024 ENST00000601374

Gene Information

Description Not Available

Gene Symbol:   PABPC1L2A
Gene Name:   poly(A) binding protein, cytoplasmic 1-like 2A
Aliases:   MGC168104, PABPC1L2B, RBM32A
RefSeq:   NC_000023.10 NT_011669.17
Ensembl:   ENSG00000186288
Entrez:   340529
UniGene:   Hs.450320
Chromosome Mapping:   Xq13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 37.610000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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