PrimePCR™ SYBR® Green Assay: ZNRF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055067

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047807
Assay Design:   exonic
Chromosome Location:   16:75141724-75141829question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000335325 ENST00000320619 ENST00000567962 ENST00000568351

Gene Information

This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment indicating that it may be involved in ubiquitin-mediated protein modification and in synaptic vessicle membranes in neurons. [provided by RefSeq Feb 2012]

Gene Symbol:   ZNRF1
Gene Name:   zinc and ring finger 1
Aliases:   DKFZp434E229, FLJ14846, MGC15430, NIN283
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000186187
Entrez:   84937
Chromosome Mapping:   16q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.480000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download