PrimePCR™ SYBR® Green Assay: SNAI3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055021

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047761
Assay Design:   exonic
Chromosome Location:   16:88744098-88744189question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000381418 ENST00000312841 ENST00000367201 ENST00000367202 ENST00000367199 ENST00000367198 ENST00000332281

Gene Information

SNAI3 is a member of the SNAIL gene family named for the Drosophila snail gene which plays roles in mesodermal formation during embryogenesis (Katoh and Katoh 2003 [PubMed 12579345]).[supplied by OMIM Apr 2009]

Gene Symbol:   SNAI3
Gene Name:   snail homolog 3 (Drosophila)
Aliases:   MGC129606, SMUC, SNAIL3, ZNF293, Zfp293
RefSeq:   NC_000016.9 NT_010542.15
Ensembl:   ENSG00000185669
Entrez:   333929
UniGene:   Hs.673548
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.560000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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