PrimePCR™ SYBR® Green Assay: PCGF3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055018

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PCGF3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PCGF3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047758
Assay Design:   exonic
Chromosome Location:   4:727464-728723question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000542440 ENST00000267197 ENST00000285238 ENST00000419774 ENST00000362003 ENST00000400151 ENST00000427463 ENST00000470161 ENST00000433814 ENST00000505655 ENST00000521023 ENST00000305877 ENST00000359540 ENST00000398512

Gene Information

The protein encoded by this gene contains a C3HC4 type RING finger which is a motif known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   PCGF3
Gene Name:   polycomb group ring finger 3
Aliases:   DKFZp686D20235, DONG1, FLJ36550, FLJ43813, MGC129615, MGC40413, RNF3, RNF3A
RefSeq:   NC_000004.11 NT_037622.5
Ensembl:   ENSG00000185619
Entrez:   10336
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.400000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download