PrimePCR™ SYBR® Green Assay: SP140L, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055007

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047747
Assay Design:   exonic
Chromosome Location:   2:231223718-231223817question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000444636 ENST00000415673 ENST00000458341 ENST00000243810 ENST00000396563 ENST00000264052 ENST00000432979 ENST00000409824 ENST00000409341 ENST00000409112 ENST00000340126 ENST00000409897 ENST00000427101 ENST00000341950

Gene Information

Description Not Available

Gene Symbol:   SP140L
Gene Name:   SP140 nuclear body protein-like
Aliases:   MGC132667
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000185404
Entrez:   93349
UniGene:   Hs.589661
Chromosome Mapping:   2q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.060000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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