This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0054982
PrimePCR™ PreAmp for SYBR® Green Assay: PURA, Human
PrimePCR™ Template for SYBR® Green Assay: PURA, Human
This gene product is a sequence-specific single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.