PrimePCR™ SYBR® Green Assay: RPS27L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054977

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RPS27L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RPS27L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047717
Assay Design:   exonic
Chromosome Location:   15:63447831-63447926question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000330964 ENST00000462430 ENST00000455271 ENST00000411926 ENST00000439025

Gene Information

This gene encodes a protein sharing 96% amino acid similarity with ribosomal protein S27 which suggests the encoded protein may be a component of the 40S ribosomal subunit. [provided by RefSeq Jul 2008]

Gene Symbol:   RPS27L
Gene Name:   ribosomal protein S27-like
Aliases:   Not Available
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000185088
Entrez:   51065
Chromosome Mapping:   15q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996700
y-intercept 37.190000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download