PrimePCR™ SYBR® Green Assay: LRRC26, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054948

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047688
Assay Design:   exonic
Chromosome Location:   9:140063466-140063583question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000371542 ENST00000316409 ENST00000397558 ENST00000465645 ENST00000570475 ENST00000257821 ENST00000359866 ENST00000382963 ENST00000422132 ENST00000361115 ENST00000256413 ENST00000382998 ENST00000295874 ENST00000474889 ENST00000343932 ENST00000174618 ENST00000502597 ENST00000256497 ENST00000297623 ENST00000447116 ENST00000429348 ENST00000367338 ENST00000362061 ENST00000300482 ENST00000397928 ENST00000397932 ENST00000300481 ENST00000429646 ENST00000367080 ENST00000303177 ENST00000519867 ENST

Gene Information

Description Not Available

Gene Symbol:   LRRC26
Gene Name:   leucine rich repeat containing 26
Aliases:   CAPC, bA350O14.10
RefSeq:   NC_000009.11 NG_011507.1 NT_024000.16
Ensembl:   ENSG00000184709
Entrez:   389816
UniGene:   Hs.669977
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.150000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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