PrimePCR™ SYBR® Green Assay: ACSM2A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054885

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ACSM2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ACSM2A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047625
Assay Design:   exonic
Chromosome Location:   16:20471601-20476931question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000329697 ENST00000565322 ENST00000565232 ENST00000567001 ENST00000568882 ENST00000566384 ENST00000569344 ENST00000414188 ENST00000324096 ENST00000544059 ENST00000318636 ENST00000574251 ENST00000576361 ENST00000417235 ENST00000573854 ENST00000219054 ENST00000575690 ENST00000571894 ENST00000396104 ENST00000424070 ENST00000536134

Gene Information

Description Not Available

Gene Symbol:   ACSM2A
Gene Name:   acyl-CoA synthetase medium-chain family member 2A
Aliases:   A-923A4.1, ACSM2, FLJ34659, MGC150530
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000183747
Entrez:   123876
Chromosome Mapping:   16p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.910000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download