This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054873
PrimePCR™ PreAmp for SYBR® Green Assay: MARCH11, Human
PrimePCR™ Template for SYBR® Green Assay: MARCH11, Human
MARCH11 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 184.108.40.206). These enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins thereby signaling their intracellular transport. March11 appears to have a role in ubiquitin-mediated protein sorting in the trans-Golgi network (TGN)-multivesicular body (MVB) transport pathway (Morokuma et al. 2007 [PubMed 17604280]).[supplied by OMIM Apr 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.