This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0054829
PrimePCR™ PreAmp for SYBR® Green Assay: SLC25A21, Human
PrimePCR™ Template for SYBR® Green Assay: SLC25A21, Human
SLC25A21 is a homolog of the S. cerevisiae ODC proteins mitochondrial carriers that transport C5-C7 oxodicarboxylates across inner mitochondrial membranes. One of the species transported by ODC is 2-oxoadipate a common intermediate in the catabolism of lysine tryptophan and hydroxylysine in mammals. Within mitochondria 2-oxoadipate is converted into acetyl-CoA.[supplied by OMIM Apr 2004]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.