PrimePCR™ SYBR® Green Assay: NTM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054805

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NTM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NTM, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047545
Assay Design:   exonic
Chromosome Location:   11:132081931-132082040question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000374791 ENST00000550167 ENST00000374786 ENST00000425719 ENST00000374784 ENST00000539799 ENST00000427481

Gene Information

This gene encodes a member of the IgLON (LAMP OBCAM Ntm) family of immunoglobulin (Ig) domain-containing glycosylphosphatidylinositol (GPI)-anchored cell adhesion molecules. The encoded protein may promote neurite outgrowth and adhesion via a homophilic mechanism. This gene is closely linked to a related family member opioid binding protein/cell adhesion molecule-like (OPCML) on chromosome 11. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2009]

Gene Symbol:   NTM
Gene Name:   neurotrimin
Aliases:   HNT, IGLON2, MGC60329, NTRI
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000182667
Entrez:   50863
Chromosome Mapping:   11q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.340000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download