PrimePCR™ SYBR® Green Assay: CAPN12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054791

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CAPN12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CAPN12, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047531
Assay Design:   exonic
Chromosome Location:   19:39221386-39221607question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000548483 ENST00000561311 ENST00000494733 ENST00000306829 ENST00000262483 ENST00000252699 ENST00000440400 ENST00000311412 ENST00000405413 ENST00000597987 ENST00000601953 ENST00000328867 ENST00000595177 ENST00000374434 ENST00000330966 ENST00000329601 ENST00000313005 ENST00000528053 ENST00000510630 ENST00000428381 ENST00000513780 ENST00000445823 ENST00000292095

Gene Information

Calpains are a family of cytosolic calcium-activated cysteine proteases involved in a variety of cellular processes including apoptosis cell division modulation of integrin-cytoskeletal interactions and synaptic plasticity (Dear et al. 2000 [PubMed 10964513]). CAPN12 belongs to the calpain large subunit family.[supplied by OMIM Mar 2008]

Gene Symbol:   CAPN12
Gene Name:   calpain 12
Aliases:   MGC20576
RefSeq:   NC_000019.9 NG_007082.2 NT_011109.16
Ensembl:   ENSG00000182472
Entrez:   147968
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.720000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download