PrimePCR™ SYBR® Green Assay: ZNF771, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054657

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF771, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF771, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047397
Assay Design:   exonic
Chromosome Location:   16:30419386-30419498question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000274712 ENST00000455860 ENST00000436311 ENST00000399344 ENST00000383194 ENST00000319296 ENST00000566625 ENST00000564550 ENST00000434417 ENST00000251413 ENST00000369044 ENST00000344683 ENST00000519480 ENST00000567107 ENST00000312015 ENST00000345070 ENST00000396879 ENST00000299989 ENST00000381761 ENST00000321596 ENST00000354127 ENST00000266000 ENST00000374542 ENST00000414083 ENST00000399060 ENST00000383062 ENST00000405315 ENST00000407032 ENST00000263273 ENST00000370904 ENST00000433482 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF771
Gene Name:   zinc finger protein 771
Aliases:   DSC43
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000179965
Entrez:   51333
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download