PrimePCR™ SYBR® Green Assay: BOLA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054547

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BOLA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BOLA1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047287
Assay Design:   exonic
Chromosome Location:   1:149871570-149871662question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000409937 ENST00000371220 ENST00000327753 ENST00000283303 ENST00000321925 ENST00000415427 ENST00000586056 ENST00000436407 ENST00000586142 ENST00000373930 ENST00000426959 ENST00000389817 ENST00000302539 ENST00000325888 ENST00000346177 ENST00000545145 ENST00000325147 ENST00000382762 ENST00000374789 ENST00000374788 ENST00000346874 ENST00000374794 ENST00000350537 ENST00000374790 ENST00000545693 ENST00000545260 ENST00000330475 ENST00000338040 ENST00000264436 ENST00000358763 ENST00000332840 ENST

Gene Information

Description Not Available

Gene Symbol:   BOLA1
Gene Name:   bolA homolog 1 (E. coli)
Aliases:   MGC75015, RP11-196G18.18, hBolA
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000178096
Entrez:   51027
Chromosome Mapping:   1p36.13-q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.360000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download