PrimePCR™ SYBR® Green Assay: SRRM3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054520

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047260
Assay Design:   exonic
Chromosome Location:   7:75915427-75915543question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000524928 ENST00000409444 ENST00000409883 ENST00000359933 ENST00000326382 ENST00000413003 ENST00000388802 ENST00000286719 ENST00000374695 ENST00000375647 ENST00000404138 ENST00000319190 ENST00000394850 ENST00000441627 ENST00000265346 ENST00000433667 ENST00000309315 ENST00000413916 ENST00000574979 ENST00000571189 ENST00000576962

Gene Information

Description Not Available

Gene Symbol:   SRRM3
Gene Name:   serine/arginine repetitive matrix 3
Aliases:   FLJ35271, FLJ37078
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000177679
Entrez:   222183
UniGene:   Hs.511025
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.690000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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