PrimePCR™ SYBR® Green Assay: GBA, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054514

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047254
Assay Design:   exonic
Chromosome Location:   1:155204702-155204791question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000368373 ENST00000327247 ENST00000427500 ENST00000536770 ENST00000428024 ENST00000574670 ENST00000572338 ENST00000602894 ENST00000602678 ENST00000602667 ENST00000602606 ENST00000602671 ENST00000602746

Gene Information

This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2010]

Gene Symbol:   GBA
Gene Name:   glucosidase, beta, acid
Aliases:   GBA1, GCB, GLUC
RefSeq:   NC_000001.10 NT_004487.19 NW_003315906.1 NG_009783.1
Ensembl:   ENSG00000177628
Entrez:   2629
UniGene:   Hs.282997
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.920000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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