PrimePCR™ SYBR® Green Assay: PHLDB3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054448

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PHLDB3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PHLDB3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047187
Assay Design:   exonic
Chromosome Location:   19:44008061-44008173question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000322641 ENST00000238146 ENST00000292140 ENST00000599242 ENST00000594808 ENST00000601646 ENST00000264018 ENST00000505450 ENST00000508167 ENST00000510224 ENST00000447510 ENST00000540805 ENST00000539194 ENST00000543373 ENST00000380574 ENST00000449793 ENST00000312221 ENST00000347936 ENST00000596625

Gene Information

Description Not Available

Gene Symbol:   PHLDB3
Gene Name:   pleckstrin homology-like domain, family B, member 3
Aliases:   FLJ40193, MGC87511
RefSeq:   NC_000019.9 NG_008141.1 NT_011109.16
Ensembl:   ENSG00000176531
Entrez:   653583
Chromosome Mapping:   19q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.300000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download