PrimePCR™ SYBR® Green Assay: WDR25, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054446

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: WDR25, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: WDR25, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047185
Assay Design:   exonic
Chromosome Location:   14:100992273-100992362question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000335393 ENST00000601861 ENST00000449408 ENST00000263253 ENST00000262848 ENST00000216271 ENST00000448072 ENST00000349505 ENST00000554998 ENST00000402312 ENST00000335290 ENST00000542471 ENST00000555201 ENST00000393561 ENST00000222399 ENST00000393560 ENST00000400374 ENST00000368868 ENST00000447402 ENST00000435071 ENST00000359428 ENST00000411637 ENST00000483497 ENST00000451030

Gene Information

Description Not Available

Gene Symbol:   WDR25
Gene Name:   WD repeat domain 25
Aliases:   MGC4645
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000176473
Entrez:   79446
Chromosome Mapping:   14q32.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.590000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download