This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0054327
PrimePCR™ PreAmp for SYBR® Green Assay: SRP72, Human
PrimePCR™ Template for SYBR® Green Assay: SRP72, Human
The SRP72 gene encodes the 72-kD subunit of the signal recognition particle (SRP) a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER) (summarized by Lutcke et al. 1993 [PubMed 8388879]). The complex consists of a 7S (or 7SL) RNA and 6 different proteins SRP9 (MIM 600707) SRP14 (MIM 600708) SRP19 (MIM 182175) SRP54 (MIM 604857) SRP68 (MIM 604858) and SRP72. The proteins are bound to the 7S RNA as monomers (SRP19 and SRP54) or heterodimers (SRP9/SRP14 and SRP68/SRP72). SRP9 and SRP14 constitute the Alu domain of 7S whereas the other 4 proteins belong to the S domain. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition translational arrest and ER membrane targeting by interaction with the docking protein.[supplied by OMIM Mar 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.