PrimePCR™ SYBR® Green Assay: APOBEC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0054259

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: APOBEC4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: APOBEC4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046998
Assay Design:   exonic
Chromosome Location:   1:183616043-183616185question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000308641

Gene Information

This gene encodes a member of the AID/APOBEC family of polynucleotide (deoxy)cytidine deaminases which convert cytidine to uridine. Other AID/APOBEC family members are involved in mRNA editing somatic hypermutation and recombination of immunoglobulin genes and innate immunity to retroviral infection. [provided by RefSeq Jul 2008]

Gene Symbol:   APOBEC4
Gene Name:   apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 4 (putative)
Aliases:   C1orf169, MGC26594
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000173627
Entrez:   403314
Chromosome Mapping:   1q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.360000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download