This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054250
PrimePCR™ PreAmp for SYBR® Green Assay: MST1, Human
PrimePCR™ Template for SYBR® Green Assay: MST1, Human
The protein encoded by this gene contains four kringle domains and a serine protease domain similar to that found in hepatic growth factor. Despite the presence of the serine protease domain the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds. [provided by RefSeq Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.