PrimePCR™ SYBR® Green Assay: ABLIM3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054222

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ABLIM3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ABLIM3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046961
Assay Design:   exonic
Chromosome Location:   5:148622079-148624551question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000326685 ENST00000309868 ENST00000506113 ENST00000504238 ENST00000508983 ENST00000517451 ENST00000356541

Gene Information

The LIM domain is a double zinc finger structure that promotes protein-protein interactions. LIM domain proteins such as ABLIM3 play roles in embryonic development cell lineage determination and cancer (Krupp et al. 2006 [PubMed 16328021]).[supplied by OMIM Mar 2008]

Gene Symbol:   ABLIM3
Gene Name:   actin binding LIM protein family, member 3
Aliases:   Not Available
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000173210
Entrez:   22885
Chromosome Mapping:   5q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.650000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download