PrimePCR™ SYBR® Green Assay: RAPH1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054219

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046958
Assay Design:   exonic
Chromosome Location:   2:204359990-204399844question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000457812 ENST00000319170 ENST00000374493 ENST00000308091 ENST00000439222 ENST00000419464 ENST00000423104 ENST00000453034 ENST00000418114 ENST00000428637 ENST00000420371 ENST00000374489 ENST00000374488

Gene Information

Description Not Available

Gene Symbol:   RAPH1
Gene Name:   Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Aliases:   ALS2CR18, ALS2CR9, KIAA1681, LPD, PREL2, RMO1, RalGDS/AF-6
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000173166
Entrez:   65059
UniGene:   Hs.471162
Chromosome Mapping:   2q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 35.870000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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