PrimePCR™ SYBR® Green Assay: ZNF738, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054186

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF738, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF738, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046925
Assay Design:   exonic
Chromosome Location:   19:21558052-21558163question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000574050 ENST00000476120 ENST00000545510 ENST00000319636 ENST00000596019 ENST00000397165 ENST00000358523 ENST00000597972 ENST00000601100 ENST00000593468 ENST00000397162 ENST00000261560 ENST00000599548 ENST00000594110 ENST00000595401 ENST00000311015 ENST00000380870 ENST00000597810 ENST00000596302 ENST00000392288 ENST00000594390 ENST00000339914 ENST00000358296 ENST00000305570 ENST00000594401 ENST00000598026 ENST00000594012 ENST00000595461 ENST00000354959 ENST00000598381 ENST00000598288 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF738
Gene Name:   zinc finger protein 738
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000172687
Entrez:   148203
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.570000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download