PrimePCR™ SYBR® Green Assay: BANP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054177

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BANP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BANP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046916
Assay Design:   exonic
Chromosome Location:   16:88008660-88008783question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000423252 ENST00000439677 ENST00000454563 ENST00000479780 ENST00000393208 ENST00000412691 ENST00000355022 ENST00000436970 ENST00000436274 ENST00000456902 ENST00000393207 ENST00000286122 ENST00000355163 ENST00000538234

Gene Information

This gene encodes a protein that binds to matrix attachment regions. The protein forms a complex with p53 and negatively regulates p53 transcription and functions as a tumor suppressor and cell cycle regulator. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2010]

Gene Symbol:   BANP
Gene Name:   BTG3 associated nuclear protein
Aliases:   BEND1, DKFZp761H172, FLJ10177, FLJ20538, SMAR1, SMARBP1
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000172530
Entrez:   54971
Chromosome Mapping:   16q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.820000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download