PrimePCR™ SYBR® Green Assay: MBOAT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054141

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MBOAT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MBOAT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046880
Assay Design:   exonic
Chromosome Location:   6:20126787-20126908question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000324607 ENST00000541730 ENST00000536798

Gene Information

MBOAT1 shares structural similarity with a superfamily of membrane-bound O-acetyltransferases that transfer organic compounds usually fatty acids (e.g. cholesterol diacylglycerol palmitoyl) onto hydroxyl groups of membrane-embedded targets (Dauwerse et al. 2007 [PubMed 17440500]).[supplied by OMIM Mar 2008]

Gene Symbol:   MBOAT1
Gene Name:   membrane bound O-acyltransferase domain containing 1
Aliases:   LPEAT1, MGC44669, OACT1, dJ434O11.1
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000172197
Entrez:   154141
Chromosome Mapping:   6p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 36.380000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download