This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054128
PrimePCR™ PreAmp for SYBR® Green Assay: QARS, Human
PrimePCR™ Template for SYBR® Green Assay: QARS, Human
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans 9 aminoacyl-tRNA synthetases specific for glutamine (gln) glutamic acid (glu) and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes mitochondria and chloroplasts in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.