PrimePCR™ SYBR® Green Assay: LRG1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054044

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046783
Assay Design:   exonic
Chromosome Location:   19:4538667-4538778question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000306390

Gene Information

The leucine-rich repeat (LRR) family of proteins including LRG1 have been shown to be involved in protein-protein interaction signal transduction and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al. 2002 [PubMed 12223515]).[supplied by OMIM Mar 2008]

Gene Symbol:   LRG1
Gene Name:   leucine-rich alpha-2-glycoprotein 1
Aliases:   FLJ45787, HMFT1766, LRG
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000171236
Entrez:   116844
UniGene:   Hs.655559
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 37.820000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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