PrimePCR™ SYBR® Green Assay: MBD3L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054016

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MBD3L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MBD3L1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046755
Assay Design:   exonic
Chromosome Location:   19:8953430-8953537question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000595891 ENST00000305625

Gene Information

This gene encodes a protein that is related to methyl-CpG-binding proteins but lacks the methyl-CpG binding domain. The protein is localized to discrete areas in the nucleus and expression appears to be restricted to round spermatids suggesting that the protein plays a role in the postmeiotic stages of male germ cell development. [provided by RefSeq Jul 2008]

Gene Symbol:   MBD3L1
Gene Name:   methyl-CpG binding domain protein 3-like 1
Aliases:   MBD3L, MGC138263, MGC138269
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000170948
Entrez:   85509
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.930000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download