PrimePCR™ SYBR® Green Assay: OSCAR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054012

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: OSCAR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: OSCAR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046751
Assay Design:   exonic
Chromosome Location:   19:54598201-54598321question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000391761 ENST00000356532 ENST00000359649 ENST00000358375 ENST00000351806 ENST00000284648 ENST00000600101 ENST00000601259 ENST00000600165 ENST00000597557 ENST00000594134 ENST00000597397 ENST00000600063 ENST00000593964 ENST00000602035 ENST00000593822 ENST00000594667 ENST00000601030 ENST00000601691 ENST00000598217 ENST00000594008 ENST00000597196 ENST00000595848 ENST00000593313 ENST00000598094 ENST00000601210 ENST00000599995 ENST00000596792 ENST00000597840 ENST00000598336 ENST00000596888 ENST

Gene Information

Osteoclasts are multinucleated cells that resorb bone and are essential for bone homeostasis. This gene encodes an osteoclast-associated receptor (OSCAR) which is a member of the leukocyte receptor complex (LRC) protein family that plays critical roles in the regulation of both innate and adaptive immune responses. Different from the other LRC members OSCAR expression is detected specifically in preosteoclasts or mature osteoclasts. OSCAR may be an important bone-specific regulator of osteoclast differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   OSCAR
Gene Name:   osteoclast associated, immunoglobulin-like receptor
Aliases:   MGC33613, PIGR3
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000170909
Entrez:   126014
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.810000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download