PrimePCR™ SYBR® Green Assay: SEMA3E, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053959

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046698
Assay Design:   exonic
Chromosome Location:   7:83047792-83095896question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000307792 ENST00000427262 ENST00000442159

Gene Information

Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities semaphorins are put into eight classes: invertebrates contain classes 1 and 2 viruses have class V and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq May 2010]

Gene Symbol:   SEMA3E
Gene Name:   sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Aliases:   KIAA0331, M-SEMAH, M-SemaK, SEMAH, coll-5
RefSeq:   NC_000007.13 NT_007933.15 NG_021242.1
Ensembl:   ENSG00000170381
Entrez:   9723
UniGene:   Hs.528721
Chromosome Mapping:   7q21.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.410000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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