PrimePCR™ SYBR® Green Assay: IFFO2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053940

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IFFO2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IFFO2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046679
Assay Design:   exonic
Chromosome Location:   1:19234884-19235030question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000368087 ENST00000466202 ENST00000324698 ENST00000434450 ENST00000374796 ENST00000297273 ENST00000246186 ENST00000373448 ENST00000373447 ENST00000449821 ENST00000312719 ENST00000265069 ENST00000492608 ENST00000348261 ENST00000565831 ENST00000358590 ENST00000220966 ENST00000433751 ENST00000377579 ENST00000455858 ENST00000273353 ENST00000542403 ENST00000597960 ENST00000222308 ENST00000257894 ENST00000544141 ENST00000365713 ENST00000371628 ENST00000439603 ENST00000304778 ENST00000422289 ENST

Gene Information

Description Not Available

Gene Symbol:   IFFO2
Gene Name:   intermediate filament family orphan 2
Aliases:   Not Available
RefSeq:   NC_000001.10 NG_012283.1 NT_004610.19
Ensembl:   ENSG00000169991
Entrez:   126917
Chromosome Mapping:   1p36.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 36.320000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download