PrimePCR™ SYBR® Green Assay: ANTXR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053909

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ANTXR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ANTXR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046648
Assay Design:   exonic
Chromosome Location:   2:69329977-69350168question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000303714 ENST00000409829 ENST00000409349 ENST00000482235

Gene Information

This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin the causative agent of the disease anthrax. The binding of the protective antigen (PA) component of the tripartite anthrax toxin to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell the other two components of anthrax toxin edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq Oct 2008]

Gene Symbol:   ANTXR1
Gene Name:   anthrax toxin receptor 1
Aliases:   ATR, FLJ10601, FLJ11298, FLJ21776, TEM8
RefSeq:   NC_000002.11 NT_022184.15 NG_012649.1
Ensembl:   ENSG00000169604
Entrez:   84168
Chromosome Mapping:   2p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.740000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download