This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053896
PrimePCR™ PreAmp for SYBR® Green Assay: COL22A1, Human
PrimePCR™ Template for SYBR® Green Assay: COL22A1, Human
COL22A1 a member of the FACIT (fibrillar-associated collagens with interrupted triple helices) subgroup of the collagen protein family specifically localizes to tissue junctions (Koch et al. 2004 [PubMed 15016833]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.