This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053867
PrimePCR™ PreAmp for SYBR® Green Assay: ROR2, Human
PrimePCR™ Template for SYBR® Green Assay: ROR2, Human
The protein encoded by this gene is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. The protein may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development. Mutations in this gene can cause brachydactyly type B a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition mutations in this gene can cause the autosomal recessive form of Robinow syndrome which is characterized by skeletal dysplasia with generalized limb bone shortening segmental defects of the spine brachydactyly and a dysmorphic facial appearance. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.