PrimePCR™ SYBR® Green Assay: FSTL5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053842

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046581
Assay Design:   exonic
Chromosome Location:   4:162306895-162307033question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000306100 ENST00000379164 ENST00000427802 ENST00000536695

Gene Information

Description Not Available

Gene Symbol:   FSTL5
Gene Name:   follistatin-like 5
Aliases:   DKFZp566D234, KIAA1263
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000168843
Entrez:   56884
UniGene:   Hs.32452
Chromosome Mapping:   4q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 37.030000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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