PrimePCR™ SYBR® Green Assay: SEPT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053809

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SEPT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SEPT2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046548
Assay Design:   exonic
Chromosome Location:   2:242274613-242275477question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000391973 ENST00000428282 ENST00000360051 ENST00000407017 ENST00000391971 ENST00000401990 ENST00000407971 ENST00000436795 ENST00000411484 ENST00000434955 ENST00000402092 ENST00000443492 ENST00000437066 ENST00000449239 ENST00000457874

Gene Information

Description Not Available

Gene Symbol:   SEPT2
Gene Name:   septin 2
Aliases:   DIFF6, KIAA0158, NEDD5, Pnutl3, hNedd5
RefSeq:   NC_000002.11 NT_005416.13
Ensembl:   ENSG00000168385
Entrez:   4735
Chromosome Mapping:   2q37

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.460000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download