PrimePCR™ SYBR® Green Assay: MOBP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053803

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MOBP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MOBP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046542
Assay Design:   exonic
Chromosome Location:   3:39543601-39543715question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000534358 ENST00000531904 ENST00000389506 ENST00000354520 ENST00000451925 ENST00000428261 ENST00000420739 ENST00000415443 ENST00000447324 ENST00000383754 ENST00000436143 ENST00000441980 ENST00000311042 ENST00000396228 ENST00000354668 ENST00000595462 ENST00000593514 ENST00000601525 ENST00000594623

Gene Information

Description Not Available

Gene Symbol:   MOBP
Gene Name:   myelin-associated oligodendrocyte basic protein
Aliases:   MGC87379
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000168314
Entrez:   4336
Chromosome Mapping:   3p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.480000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download