PrimePCR™ SYBR® Green Assay: NKIRAS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053793

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NKIRAS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NKIRAS2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046532
Assay Design:   exonic
Chromosome Location:   17:40173602-40174418question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000544817 ENST00000308230 ENST00000382179 ENST00000528710 ENST00000526678 ENST00000531197 ENST00000528841 ENST00000308219 ENST00000388759 ENST00000443659 ENST00000421515 ENST00000425478 ENST00000437230 ENST00000412028 ENST00000415901 ENST00000416026 ENST00000312865 ENST00000538643 ENST00000322773 ENST00000325718 ENST00000305231 ENST00000508661 ENST00000262577 ENST00000585955 ENST00000307641 ENST00000393885 ENST00000479407 ENST00000393880 ENST00000393881 ENST00000462043 ENST00000449471 ENST

Gene Information

Description Not Available

Gene Symbol:   NKIRAS2
Gene Name:   NFKB inhibitor interacting Ras-like 2
Aliases:   DKFZp434N1526, KBRAS2, MGC74742, kappaB-Ras2
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000168256
Entrez:   28511
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.460000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download