This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053786
PrimePCR™ PreAmp for SYBR® Green Assay: MAP4K2, Human
PrimePCR™ Template for SYBR® Green Assay: MAP4K2, Human
The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues its expression in lymphoid follicles is restricted to the cells of germinal centre where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2) which is involved in the activation of MAP3K1/MEKK1. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.