PrimePCR™ SYBR® Green Assay: TMIGD2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053740

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046479
Assay Design:   exonic
Chromosome Location:   19:4292788-4294578question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000339235 ENST00000333467 ENST00000402182 ENST00000407298 ENST00000543139 ENST00000301332 ENST00000263253 ENST00000359963 ENST00000319363 ENST00000549982 ENST00000374690 ENST00000435347 ENST00000253083 ENST00000373146 ENST00000373141 ENST00000373142 ENST00000373144 ENST00000291839 ENST00000274867 ENST00000398904 ENST00000538976 ENST00000239151 ENST00000439268 ENST00000340578 ENST00000595645 ENST00000301272 ENST00000600114 ENST00000600349 ENST00000420338 ENST00000327535 ENST00000327575 ENST

Gene Information

Description Not Available

Gene Symbol:   TMIGD2
Gene Name:   transmembrane and immunoglobulin domain containing 2
Aliases:   MGC23244
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000167664
Entrez:   126259
UniGene:   Hs.263928
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.760000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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