PrimePCR™ SYBR® Green Assay: LAIR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053730

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LAIR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LAIR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046469
Assay Design:   exonic
Chromosome Location:   19:54872647-54872747question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000600252 ENST00000391743 ENST00000348231 ENST00000391742 ENST00000434277 ENST00000474878 ENST00000438193 ENST00000313038 ENST00000574314 ENST00000575213 ENST00000573407 ENST00000576124 ENST00000572189 ENST00000574643 ENST00000571037 ENST00000600145 ENST00000598638 ENST00000576928

Gene Information

The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells including NK cells T cells and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. [provided by RefSeq Jul 2008]

Gene Symbol:   LAIR1
Gene Name:   leukocyte-associated immunoglobulin-like receptor 1
Aliases:   CD305, LAIR-1
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000167613
Entrez:   3903
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.980000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download