PrimePCR™ SYBR® Green Assay: TMC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053729

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TMC4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TMC4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046468
Assay Design:   exonic
Chromosome Location:   19:54675670-54675767question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000328086 ENST00000341640 ENST00000378740 ENST00000463946 ENST00000338516 ENST00000394303 ENST00000579828 ENST00000373986 ENST00000361209 ENST00000361477 ENST00000313400 ENST00000373996 ENST00000308527 ENST00000372922 ENST00000340840 ENST00000354325 ENST00000541110 ENST00000372917 ENST00000301187 ENST00000376591 ENST00000446291 ENST00000310917 ENST00000344408 ENST00000344938 ENST00000343638 ENST00000302904 ENST00000271628 ENST00000273067 ENST00000454610 ENST00000258416 ENST00000582851 ENST

Gene Information

Description Not Available

Gene Symbol:   TMC4
Gene Name:   transmembrane channel-like 4
Aliases:   MGC39329
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000167608
Entrez:   147798
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.550000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download