PrimePCR™ SYBR® Green Assay: IGF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053707

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046446
Assay Design:   exonic
Chromosome Location:   11:2153942-2154071question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000382103 ENST00000302922 ENST00000300632 ENST00000337883 ENST00000418738 ENST00000381389 ENST00000381392 ENST00000434045 ENST00000416167 ENST00000381406 ENST00000381395

Gene Information

This gene encodes a member of the insulin family of polypeptide growth factors which are involved in development and growth. It is an imprinted gene expressed only from the paternal allele and epigenetic changes at this locus are associated with Wilms tumour Beckwith-Wiedemann syndrome rhabdomyosarcoma and Silver-Russell syndrome. A read-through INS-IGF2 gene exists whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]

Gene Symbol:   IGF2
Gene Name:   insulin-like growth factor 2 (somatomedin A)
Aliases:   C11orf43, FLJ22066, FLJ44734, IGF-II, PP9974
RefSeq:   NC_000011.9 NG_008849.1 NT_009237.18
Ensembl:   ENSG00000167244
Entrez:   3481
UniGene:   Hs.272259
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.420000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download