PrimePCR™ SYBR® Green Assay: CHRFAM7A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053659

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CHRFAM7A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CHRFAM7A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046398
Assay Design:   exonic
Chromosome Location:   15:30654861-30659624question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000331242 ENST00000413276 ENST00000394703 ENST00000454008 ENST00000414194 ENST00000299847 ENST00000401522 ENST00000397827 ENST00000454250 ENST00000306901 ENST00000455693 ENST00000221249 ENST00000545201 ENST00000414982 ENST00000450331 ENST00000600737 ENST00000537753 ENST00000600904 ENST00000602195

Gene Information

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The family member CHRNA7 which is located on chromosome 15 in a region associated with several neuropsychiatric disorders is partially duplicated and forms a hybrid with a novel gene from the family with sequence similarity 7 (FAM7A). Alternative splicing has been observed and two variants exist for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel. Although current evidence supports transcription of this hybrid gene translation of the nicotinic acetylcholine receptor-like protein-encoding open reading frames has not been confirmed. [provided by RefSeq Jul 2008]

Gene Symbol:   CHRFAM7A
Gene Name:   CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion
Aliases:   CHRNA7, CHRNA7-DR1, D-10, MGC120482, MGC120483
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000166664
Entrez:   89832
Chromosome Mapping:   15q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.060000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download