This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053604
PrimePCR™ PreAmp for SYBR® Green Assay: TAF1D, Human
PrimePCR™ Template for SYBR® Green Assay: TAF1D, Human
TAF1D is a member of the SL1 complex which includes TBP (MIM 600075) and TAF1A (MIM 604903) TAF1B (MIM 604904) and TAF1C (MIM 604905) and plays a role in RNA polymerase I transcription (Wang et al. 2004 [PubMed 15520167]; Gorski et al. 2007 [PubMed 17318177]).[supplied by OMIM Jun 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.