PrimePCR™ SYBR® Green Assay: TAF1D, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053604

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046343
Assay Design:   exonic
Chromosome Location:   11:93471379-93471510question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000448108 ENST00000532455

Gene Information

TAF1D is a member of the SL1 complex which includes TBP (MIM 600075) and TAF1A (MIM 604903) TAF1B (MIM 604904) and TAF1C (MIM 604905) and plays a role in RNA polymerase I transcription (Wang et al. 2004 [PubMed 15520167]; Gorski et al. 2007 [PubMed 17318177]).[supplied by OMIM Jun 2009]

Gene Symbol:   TAF1D
Gene Name:   TATA box binding protein (TBP)-associated factor, RNA polymerase I, D, 41kDa
Aliases:   JOSD3, MGC5306, RAFI41, TAF(I)41
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000166012
Entrez:   79101
UniGene:   Hs.355750
Chromosome Mapping:   11q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.970000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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